Inhaltsangabe1. BioBrick Assembly Standards and Techniques and Associated Software ToolsGunvor Røkke, Eirin Korvald, Jarle Pahr, Ove Øyås, and Rahmi Lale 2. Plasmid Construction by SLIC or Sequence and Ligation Independent CloningRyan E. Hill, Julian J. Eaton-Rye 3. Quick and Clean CloningFrank Thieme and Sylvestre Marillonnet 4. Hierarchical Ligation-Independent Assembly of PCR FragmentsJonathan L. Schmid-Burgk, Zhen Xie, and Yaakov Benenson 5. USER-Derived Cloning Methods and Their Primer DesignBo Salomonsen, Uffe H Mortensen, Barbara A Halkier 6. Application of the Restriction-Free (RF) Cloning for Multi-Components AssemblyYoav Peleg and Tamar Unger 7. A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) PlatformAriel Erijman, Julia M. Shifman, and Yoav Peleg  8. Circular Polymerase Extension CloningJiayuan Quan and Jingdong Tian 9. Golden Gate Cloning Carola Engler and Sylvestre Marillonnet 10. Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning StrategyAlejandro Sarrion-Perdigones, Jorge Palaci, Antonio Granell, Diego Orzaez 11. FX cloning: A Simple and Robust High-Throughput Cloning Method for Protein ExpressionEric R. Geertsma 12. Minimum GC-rich Sequences for Overlap Extension PCR and Primer AnnealingMikiko Nakamura, Ayako Suzuki, Hisashi Hoshida, and Rinji Akada 13. Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCRChun You and  Y.-H. Percival Zhang 14. Combinatorial Assembly of Clone Libraries Using Site-Specific RecombinationVanessa E. Wall, Leslie A. Garvey, Jennifer L. Mehalko, Lauren V. Procter, and Dominic Esposito 15. Application of In-Fusion Cloning for the Parallel Construction of Escherichia coli Expression Vectors Louise E. Bird, Heather Rada, John Flanagan, Jonathan M. Diprose, Robert J.C. Gilbert, Raymond J. Owens 16. SeamLess Ligation Cloning Extract(SLiCE)Cloning MethodYongwei Zhang, Uwe Werling, and Winfried Edelmann 17. j5 DNA Assembly Design AutomationNathan J. Hillson 18. FASTPCR Software for PCR, in silico PCR, and Oligonucleotide Assembly and AnalysisRuslan Kalendar, David Lee, and Alan H. Schulman